Established in 2008
Evolving young minds to serve society
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Established in 2008
Evolving young minds to serve society

BIOTECHNOLOGY

Biotechnology, the technology harnessing cellular and bio-molecular processes to develop procedures and products, has amalgamated biology and technology. It has the fundamental aim to improve the quality of life. Today Biotechnology finds its wide applications and has potential impact on virtually all domains of human welfare. The department incessantly aims to train students by imparting them latest industry required knowledge in the fields of Biotechnology and other allied areas.

The Department of Biotechnology, B. N. College of Engineering and Technology, Lucknow is offering academic program B. Tech (Biotechnology) since 2015. We follow the curriculum of APJ AKTU orienting towards imparting skills in addition to the in-depth coverage of the core discipline. Being a highly refined and sophisticated discipline, the Department of Biotechnology had experienced faculty with a rich and varied teaching and research exposure. Many of them have doctoral research experience from reputed national institutions.

Besides encouraging students to become proficient in their academic arena, the Department of Bio-technology motivates them to participate in various personality enhancing activities. The student club Bio-Sapiens regularly conducts curricular and co-curricular activities and newsletter Aarohan provides the information related to technical, cultural and literary competitions at both intra and inter University level. Professionals from industry and national research laboratories, visit the department to give guest lectures and seminars on the latest developments and industry practices in Biotechnology and allied domain. Biotechnology engineers can easily make a career in Bioinformatics, Pharma, Mushroom cultivation etc. for which the department conducts value added programmes to prepare the students for their prospective careers.

Dr. Pankaj Verma

(Head, Department of Biotechnology)         

Department of biotechnology in BNCET was established in 2013. All the basic infrastructural facilities needed for conducting teaching and research in different areas of Biotechnology have been developed in the department, efforts are being made to bring the department under the ambit of Department of Biotechnology (DBT), Government of India, so that better facilities are ensured to our present and prospective students. Besides guiding students to excel in their academics, the Department strives hard to uplift budding scientists and encourages them to participate in activities aimed at nourishing their inherent talent. Regular conferences, extension lectures and training workshops are arranged at the department to give our students the necessary exposure to compete at the national level.

To produce quality Biotech engineers tailored to promote innovation and dissemination of knowledge in order to benefit the environment and country as well as to gain excellence in various spheres of Biotechnology fortified to create graduates who endeavor societal welfare.

M1.     To incubate innovative ideas at innovations hubs and center of excellences to design and develop products/applications followed by their up scaling and industrial tie up.

M2.     To integrate the traditional and modern Biotechnology and educate students how they can provide benefits to industry, society and country by upgrading their skills with the help of state-of-the-art research labs and nurtured academia.

M3.     To participate in various academic/sports/social activities to develop their interpersonal skills of communication, team work and also learn moral/ethical practices.

PEO1. To translate Biotechnological principles into practical applications by promoting multidisciplinary research in house/government or private research labs.

PEO2. To enrich abilities for understanding, learning and adopting any new technology which is helpful in updating their pre-existing skill sets towards benefits of all stakeholders.

PEO3. To develop confidence of speaking at mass level, presentation of ideas and skill to discuss any scientific issue with scholars and laureates.

PSO1.  Able to design protocols for synthesizing any product, downstream processing including quality control which can be easily scaled up fulfilling market expectations.

PSO2.  Learn various concepts, principles, methodologies and precautions of different tools & techniques covering every aspect of Biotechnology domain used in research labs as well as industry level.

PSO3.  Learn necessary ethics required for enhancement of academic and industrial practices with societal awareness which help in higher education and professional life.

  • PO1- Engineering knowledge: Apply the knowledge of mathematics, science, engineering fundamentals, and an engineering specialization to the solution of complex engineering problems.
  • PO2-Problem analysis: Identify, formulate, review research literature, and analyze complex engineering problems reaching substantiated conclusions using first principles of mathematics, natural sciences, and engineering sciences.
  • PO3-Design/development of solutions: Design solutions for complex engineering problems and design system components or processes that meet the specified needs with appropriate consideration for the public health and safety, and the cultural, societal, and environmental considerations.
  • PO4-Conduct investigations of complex problems: Use research-based knowledge and research methods including design of experiments, analysis and interpretation of data, and synthesis of the information to provide valid conclusions.
  • PO5-Modern tool usage: Create, select, and apply appropriate techniques, resources, and modern engineering and IT tools including prediction and modeling to complex engineering activities with an understanding of the limitations.
  • PO6-The engineer and society: Apply reasoning informed by the contextual knowledge to assess societal, health, safety, legal and cultural issues and the consequent responsibilities relevant to the professional engineering practice.
  • PO7-Environment and sustainability: Understand the impact of the professional engineering solutions in societal and environmental contexts, and demonstrate the knowledge of, and need for sustainable development.
  • PO8-Ethics: Apply ethical principle s and commit to professional ethics and responsibilities and norms of the engineering practice.
  • PO9-Individual and team work: Function effectively as an individual, and as a member or leader in diverse teams, and in multidisciplinary settings.
  • PO10-Communication: Communicate effectively on complex engineering activities with the engineering community and with society at large, such as, being able to comprehend and write effective reports and design documentation, make effective presentations, and give and receive clear instructions.
  • PO11-Project management and finance: Demonstrate knowledge and understanding of the engineering and management principles and apply these to one’s own work, as a member and leader in a team, to manage projects and in multidisciplinary environments.
  • PO12-Life-long learning: Recognize the need for, and have the preparation and ability to engage in independent and life-long learning in the broadest context of technological change.

 
Sr. No Name of Lab Name of the Officer-Incharge Major Equipoments List of Experiments
1 Cell and Microbiology Lab Dr. Rishi Kr. Verma Microscope, Autoclave, Laminar Air Flow 1I.noculation of agar slants, agar plate and nutrient broth (Incubators, Water bath, Laminar hood, dry heat sterilizer i.e. bead sterilizer) 2. Culture of microorganisms using various techniques. (Shakers i.e. Cooling and Open shaker). 3. Simple and differential staining procedures, endospore staining, flageller staining, cell wallstaining, capsular staining, negative staining. (Moist chambers, spirit lamps, slides, loops & microscopes, haemocytometer)
2 Genetics & Molecular biology Lab Mr. Rajiv Rai Electrophoresis Unit, Centrifuge 1. How to calculate genetics and allelic frequencies numeric problem analysis. 2. Isolation of Plasmid DNA 3. Isolation of Plant DNA 4. Estimation of DNA content in the given sample by spectrophotometer 5. Determination of Tm of DNA. 6. Isolation of bacterial genomic DNA.
3 Microbiology & Immunology Lab Mr. Sunil Kr. Verma Laminar Air Flow Hood, Heating Oven, Autoclave, Incubator 1. Preparation of nutrient agar slants, plates and nutrient broth and their sterilization. (Microwave Oven, Heating mantles, Fridge, Heating Oven, Tube racks) 2. Inoculation of agar slants, agar plate and nutrient broth (Incubators, Water bath, Laminar hood, dry heat sterilizer i.e. bead sterilizer) 3. Culture of microorganisms using various techniques. (Shakers i.e. Cooling and Open shaker). 4. Simple and differential staining procedures, endospore staining, flageller staining, cell wallstaining, capsular staining, negative staining. (Moist chambers, spirit lamps, slides, loops & microscopes, haemocytometer) 5. Bacterial colony counting. (Moist chambers, spirit lamps, slides, loops & microscopes, haemocytometer) 6. Observation of different vegetative, capsular and spore forms of bacteria & fungus under various microscopes. 1. How to calculate genetics and allelic frequencies numeric problem analysis. 2. Isolation of Plasmid DNA 3. Isolation of Plant DNA 4. Estimation of DNA content in the given sample by spectrophotometer 5. Determination of Tm of DNA. 6. Isolation of bacterial genomic DNA.
4 Biochemistry Md. Haseeb Khan Ph meter 1. Preparation of solutions: 1)percentage solutions, 2) molar solutions, 3) normal solutions 2. Standardization of pH meter, preparation of buffers, emulsions. 3. Spectroscopy: determination of absorption maxima (λmax) of a given solution 4. Titration of weak acid-weak base 5. Quantitative estimation of carbohydrates 6. Distinguish reducing and non-reducing sugars 7. Quantitative estimation of proteins
5 Bioinformatics Dr. Prashant Kumar Computer, Projector 1. Get five nucleotide and five protein sequences in FASTA format from NCBI and EMBL. 2. To find out five similar sequences for any nucleotide and protein query sequence using BLAST and FASTA. 3. Access and use of different online nucleotide and protein alignment tools (Pairwise and Multiple sequence alignment). 4. Genes and Exons identification related search for a given genome sequence in order to predict the gene. 5. ORF prediction in the given nucleotide sequence. 6. Secondary structure prediction for given amino acid sequences of a given protein using Chou Fasman, GOR method and Neural Network method. 7. Visualize tertiary structure of any given protein sequence. 8. Carry out the alignment of genomes of given organisms.
6 Bioprocess Engineering Lab-I Ms. Shivanisingh Hot plate magnetic stirrer 1. Determine the growth patterns and specific growth rate of E.coli 2. Determine the effect of peptone concentration on E.coli. growth 3. Determination of specific thermal death rate constant (kd) for E.Coli 4. Determine the effects of temperature & pH on Pseodomonasputida 5. Upstream and Downstream of bioprocess for the production of Citric acid by Aspergillusniger
7 Techniques in Biotechnology Lab Dr. Rishi Kr. Verma Microscopes 1. Precision, accuracy and validity of an experiment. Analysis and presentation of data. 2. Absorption maxima– change in absorbance in potassium permanganate with wavelength. 3. Verification of Beer-Lambert’s law-using UV-Vis spectrophotometer. 4. To study the structure & function of basic microscope. 5. To study the paper chromatography for plant pigments 6. To study the thin layer chromatography for Amino acids 7. To study the column chromatography for separates the mix samples. 8. To study the analysis of DNA by agarose gel electrophoresis method. 9. To study the analysis of protein by SDS- PAGE method
8 Enzyme Engineering Lab Ms. Kavita Patel Laminar Air Flow Hood 1. Production of commercially important enzymes from microbial sources. 2. Isolation of alpha amylase from plant source 3. Determination of enzyme activity and specific activity. 4. Partial purification of isolated enzymes. 5. Method of checking the purity of the enzyme -SDS-PAGE 6. Characterization of enzymes-effect of pH , temperature and inhibitors on enzyme activity etc. 7. Identification of Enzyme by different assay
9 Genetic Engineering Lab Mr. Rajiv Rai Microscopes 1. Estimation of DNA by diphenylamine reaction 2. Determination of RNA by orcinol method 3. To isolate plant DNA using CTAB. 4. Elution of plant DNA fragment from agarose gel
10 Fermentation Biotechnology Lab Ms. Shivanisingh Laminar Air Flow, Incubator, Autoclave, Ph meter 1. Fermentative production of Penicillin Antibiotics using Peniciliumchrysogenum. 2. To study the induction effect of β-galactosidase enzyme in E.coli. 3. Upstream and Downstream of bioprocess for the production of Citric acid by Aspergillus niger 4. Citric acid production from whey with glucose as supplementary carbon source by Aspergillusniger 5. Microbial production of citric acid by solid state fermentation process. 6. Microbial production of enzymes by (a) solid state and (b) submerged fermentation.
11 Nano-Biotechnology Lab Md Haseeb khan Incubator 1. Introduction to nanoscience and nanobiotechnology (Size comparative analysis) 2. Synthesis and characterization of carbon nanotubes from carbon source. 3. Chemical synthesis of silver and gold nanoparticles; UV-Visible absorption of the colloidal solution and estimation of size by curve fitting. 4. Biological synthesis of silver and gold nanoparticles; UV-Visible absorption of the colloidal solution and estimation of size by curve fitting. 5. Metal nanoparticles toxicity estimation in percentage as in vitro
12 Plant Biotechnology Lab Mr. AmalBhardwaj Centrifuge, Incubator, Magnetic stirrer 1. Preparation of Stocks solution for plant tissue culture media. 2. Preparation of MS/B5 medium (semi-solid) and sterilization. 3. Explant selection, preparation and surface sterilization. 4. To learn culturing, sub culturing and maintenance using selected explants. 5. Initiation of in vitro cultures through axillary bud induction 6. Initiation of callus cultures from different explants.
13 Environmental Biotechnology Lab Mr. AmalBhardwaj Laminar Air Flow Hood, Heating Owen 1.Calculation  of  BOD 2. Calculation of COD. 3. Calculation of coliform in sewage water. 4.Treatment of sewage water
14 Bio-separation & Down Stream Processing Lab Mr. Sunil Kr. Verma Autoclave, Fractional distillation column 1. Characteristics of Bioproducts: Flocculation and conditioning of broth 2. Mechanical separation: Filtration and Centrifugation 3. Cell disruption 4. Membrane based eparation 5. Protein precipitation and its eparation: Aqueous two phase extraction, Ultra filtration and Adsorption 6. Chromatography eparation based on size, charge, hydrophobic interaction 7. Gel analysis/ assay for dialysed product  
 
  1. Publication in National/International Journal

Sr. No.

Faculty Name

Publication (National)

Publication

(International)

1.

Dr. Pankaj Verma

3

4

2.

Dr. Rishi Kumar Verma

3

3

3.

Dr. Prashant Kumar

0

4

4.

Ms.Shivani Singh

0

1

5.

Sunil Kumar Verma

0

2

6.

AmalBhardwaj

0

1

7.

Mr. Md. Haseeb

0

1

8

Ms. RiyaAgarwal

0

1

9

Mr. Rajiv Rai

2

0

 

  1. Publication in National/International Conference

Sr. No.

Faculty Name

Publication (National)

Publication

(International)

1.

Dr. Pankaj Verma

2

0

2.

Dr. Rishi Kumar Verma

2

0

2

Dr. Prashant Kumar

2

1

4

Ms.Shivani Singh

5

2

5

Ms. Kavita Patel

2

1

 

  1. Best Projects

Session:2017-19

Sr. No

Name of Students

(Roll No.)

Name of Guide

Project Title

Brief Description

1

Swati singh

1443154018

Msshaziawahid

Microglia activation followed by Japanese encephalitis

Japanese encephalitis (JE) is an acute and uncontrolled inflammatory disease of central nervous system in humans especially affecting children.virus induced inflammation contributes to disease severity by inducing neuronal cell death (chen CJ et al 2010), inhibiting the proliferation and differentiation of neural progenitors and disrupting the blood brain barriers.

2

Aditipathak

1443154001

Mr. Haseeb

Observation on IgG ELISA for diagnosis of intra-abdominal Echinococcosis at a tertiary care level super specialty institute in north India.

Urinary tract infections are most commons. They are mostly predominated by gram negative organism. The importance of knowing local antimicrobial susceptibility to direct empirical antibiotic therapy cannot be overemphasized.

3

Nidhishribalarajvanshi

1443154010

Mr. Haseeb

Detection of HIV by ELISA technique in a tertiary care centre.

. Primary diagnosis HIV infection is commonly accomplished by serological via detection of HIV antibody using a screening enzymes immunoassay followed by a subsequent confirmatory western blot test.

Session:2018-19

Sr. No

Name of Students

(Roll No.)

Name of Guide

Project Title

Brief Description

1

Aayushikatiyaar

Md. Haseeb Khan

.Aanalysis of mutagenic chemicals present in branded and homemade pickle along with its quality analysis

The toxic chemical compounds present in food as preservatives cause irritation of the skin and mucous membranes, lung damage, upset the ionic equilibrium in our bloodstream and degeneration of nervous system tissue (Campbell et..al 2001).Various biochemical test, mutagenic chemical identification test and qualitative test were conducted to test quality and efficiency of pickle preservation

2

Bhawana Singh Bharti

Dr. Pankaj Verma

Survivin mediated synthesis of gold nano-particles

Gold nana-particles are widely used in many fields as preferred materials for their unique optical and physical properties such as surface Plasmon oscillations for labeling, imaging and sensing .

Gold nano-particles can be prepared and conjugated with many functionalizing agents such as polymers, surfactants, ligands, dendrimers , drugs , DNA, RNA, proteins , peptides and oligonucleotides

Session:2019-20

Sr. No

Name of Students

(Roll No.)

Name of Guide

Project Title

Brief Description

1

Mahfooz

Dr. Pankaj Verma

Bio-Synthesis of Silver and Magnesium Nano-Participle from Cadmba.

Synthesis of silver and magnesium and particles by Cadamba Extract and to study its anti-microbial and anti-fungal acitivity.

2

Vidushi

Dr. Rishi Kr. Verma

Bio-Synthesis of Silver and Magnesium Nano-Participle from Cadmba.

Synthesis of silver and magnesium and particles by Cadamba Extract and to study its anti-microbial and anti-fungal acitivity.

3

Saumya Gupta

Dr. Pankaj Verma

“TRICHODERMA VIRIDE: A FUNGUS HAVING ANTIFUNGAL AND ANTIBACTERIAL PROPERTIES

The chronological development of Trichoderma spp., a strategy of activity against different biological agents, probable applications, and unusual mass production techniques are seen.